Ultra Luciferase Detection?Kit
CBPH0001
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產(chǎn)品描述
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| Ultra Luciferase Detection Kit | ||||
| Descripition |
Ultra luciferase detection kit provides an assay reagent that generates ultra-bright signal in the presence of firefly luciferase. The assay reagent is prepared by mixing substrate and cell lysis buffer
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| 產(chǎn)品組分 | ||||
| 貨號 | CBPH0001-1 | CBPH0001-2 | CBPH0001-3 | CBPH0001-4 |
| 規(guī)格 | 100 points | 1000 points | 5000 points | 10000 points |
| 組分A: Cell lysis buffer |
1 bottle 10 mL/bottle |
2 bottles 50 mL/bottle |
5×CBPH0001-2-A | 10×CBPH0001-2-A |
| 組分B: Substrate 50X |
1 vial 200μL/vial |
2 vials 1 mL/vial |
5×CBPH0001-2-B | 10×CBPH0001-2-B |
| 數(shù)據(jù)展示 | ||||
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1.Cobioer-UItra VS Bright-glo
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Figure 2. TNF α Dose Response Curve.
Figure 3. NF-KB Hek293 cells. |
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2.凍融測試
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Figure 4. TNF α Dose Response Curve. |
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3.定量和裂解能力測試
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Figure 5. Ultra vs Bright-glo. |
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| Protocol | ||||
| 1.檢測試劑的準備 | ||||
| 使用前底物和細胞裂解液在4 ℃或室溫下溶解。開蓋前建議離心，集中液體到瓶底。按照1:50的體積比將組分B加入到組分A中，充分混勻配置所需量的檢測試劑。 | ||||
| 2.螢火蟲螢光素酶的檢測 | ||||
| ·檢測試劑和測試細胞樣本平衡至室溫。 | ||||
| ·向測試細胞樣本中加入等體積的檢測試劑。 | ||||
| 96孔板檢測體系，100μL培養(yǎng)基中加入100μL檢測試劑。 | ||||
| ·在測量前1200轉(zhuǎn)震蕩5分鐘，充分震蕩會使效果更好。 | ||||
| 3. Notes | ||||
| ·不同批號試劑盒中的試劑不可混合使用。 | ||||
| ·不透明板子用于檢測，白色不透明的96孔檢測效果更好。 | ||||
| Product Information | ||||
| Intended Use | Research Use Only | |||
| Unit Size | 100 points/1000 points/5000 points/10000 points | |||
| Storage | -80℃~-20℃ | |||
| Expiry | 12 months from the date of manufacture | |||
